Composite

Part:BBa_K2206007:Design

Designed by: Sammy Lovat   Group: iGEM17_CLSB-UK   (2017-10-02)


Toehold switch for hsa-miR-27b-3p with GFPmut3b and inducible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1932
    Illegal SapI site found at 961


Design Notes

No terminators are needed as the pSB1C3 plasmid contains terminators.

A non-leaky inducible promoter is needed to amplify the constructs in E.coli as quite a high concentration of the transcripts is toxic to E.coli.

There is a scar between the promoter and the toehold switch, but there is no scar between the toehold switch and the reporter protein.

Source

This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.

References